Active low is a setting in Mach, it is either checked or not (in your case not). Barring a bunch of knowledge, check it and see if the response is different. My suspicion is that your steps per unit is too small. Yours is set to 1000. Most steppers have 200 units per rev with 10x micro stepping equates to 2000 steps per unit. Plenty to be confused about and I am sure someone will point out the ignorance in my process too. I have never caused a problem by checking active low or not. It might not have solved my problem and I return it to its original setting. I believe it refers to the switch going to ground or +5v.
A lazy man does it twice.