Looking for some help with motor tuning.

Specs:
Nema 34 3.5 amp 1600oz/in motors
DM860A drivers / 2000step/rev
BL-MACH-V1.1 ST-V2 Breakout Board
16mm ball screws 5mm pitch - 10160 steps/unit (inch)
Mach3 Software - Version R3.043.062
Windows 7 Pro OS

When I open the motor tuning screen and tune the motors to 50ipm and velocity of 10 in/sec/sec. all the axis's move smooth and motors sound fine. After I save the settings and exit and move any axis the motors sound choppy and not at all smooth. I have tried adjusting everything ipm, velocity, kernel, nothing has helped. Any help or suggestions would be greatly appreciate. Thanks for your support.