Port#1 on the UC300ETH is input port where pins 2 to 9 are inputs. On the UC100 those pins are outputs.
That is the reason why it does not work for you, because you trying to use input pins for the step/dir, but they are output functions.
Connect your BOB to port#2 on the UC300ETH that is normal port which has the same pinout as the UC100.

For the pin out of the UC300ETH port check the users guide or the diagnostics page in the UCCNC that also shows which pins are in and outputs.