Looks like it should work out of the box. Nice looking unit.
Steppers lock up when powered up. -- normal
Are you using a PC? With a parallel port? YES! PCI card. That's good. Configured correctly? Port number recognised by Windows device manager?
Some sort of a parallel port adapter? -- Typically only a smoothstepper or similar will work on USB
USB? - don't go there unless you already have a smoothstepper.
USB to parallel WILL NOT WORK with Mach3.
A laptop? -- now there is a problem. -- Use a PC to get started with.
Have you run drivertest.exe - No?
Read the Mach3 manual? At least read the installation section.
You need to setup all the pins/ports etc in MAch3 unless it came with a setup file 'somename.xml'
If the setup file exists, then use then put it in the Mach3 folder, then run Mach3 and select it as the profile.
Super X3. 3600rpm. Sheridan 6"x24" Lathe + more. Three ways to fix things: The right way, the other way, and maybe your way, which is possibly a faster wrong way.