Thank you for hanging in there with me! When I was setting up my AXIS I did exactly like you had said, one axis at a time with the other 2 turned off. I would then restart Mach3 and move on to the other two in exactly the same method. It worked and I was thrilled. I guess I failed to mention I really did things slowly, but then I'd find new issues the next time I'd come back to my machine. For instance, this morning I found a build sheet on a BLACKFOOT CNC and it matched all my components perfectly, the correct NEMA motors and microstep drivers, chain & gears. I used their MOTOR TUNING guide and I had my 3 axis moving positive & negative and no more jerky motions. I ran the sample ROADRUNNER G-Code twice (with a magic marker in the router chuck) and measured it to make sure it turned out the correct size. Everything looked real good. I turned off my computer & CNC machine and returned 5 hours later and again, my Y axis won't budge. Mach3 recognizes my arrow keys, but no motor movement at all. So, I started from the beginning all over again. I isolated the axis on the Ports & Pins and never was able to get it moving again. I wish I could post some pictures, but I can't figure out how to do it on this computer, it won't recognize my phone. At this point I don't believe it's a MACH3 issue or loose wire. I know it must seem like I'm not a good listener, (at least that what my wife tells me) but I do fallow the advise you give and so far it's all worked, thank you. Do you have thoughts on why my axis would work fine, then just be unresponsive? It's getting late, tomorrow I'll install with a new profile and try again.