On my machine when I got it, I found three places for backlash on the original lead screws.
1. Where the handwheels fit on to the lead screw, there was slop between the back side and the thrust bearings in the bearing mount.
2. As mentioned, the split nut may need adjusting
3. I found particularly on the X axis the mounting bolts would actually work loose and allow the entire mount to move (admittedly this was after I had cnc'd it and had a few hard crashes:{(, but the potential is still there.
Art
AKA Country Bubba (Older Than Dirt)