Thanks steryd,
I think that my PC Mach3 has lost it's machine data (Length of axis, pulse/distance etc)
My next step was to test the steppers with external Pulse/Direction, they have a good 45v supply.
I purchased the machine recently as a "none runner - Axis" I think that the original reason for the failure was loss of machine data, which will have stopped mach3 referencing and therefore the axes working, but since then the previous owner has waded in with many PCB changes, linking out the axes supply 10A fuse etc, etc.
Regards, Dai.