Hmm, that is weird. What could have changed since it worked last time?
More tests to isolate the problem:
* Try GDflasher utily to test communication to drives. Also you may try flashing firmware to drives, it will reset all saved settings (if that would help).
* Unplug all unnecessary USB devices from computer. If some other device has FTDI brand chip, it may get mixed with TTL232 cable.
* Try GDtool on another computer (just in case it's software/driver/windows issue)
Please let me know how the tests went.